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- Challenges and Chances: A Review of the 1st Stem Cell Community Day
- Summertime, and the Livin’ Is Easy…
- Follow-on-Biologics – More than Simple Generics
- Bacteria Versus Body Cells: A 1:1 Tie
- Behind the Crime Scene: How Biological Traces Can Help to Convict Offenders
- Every 3 Seconds Someone in the World Is Affected by Alzheimer's
- HIV – It’s Still Not Under Control…
- How Many Will Be Convicted This Time?
- Malaria – the Battle is Not Lost
- Physicians on Standby: The Annual Flu Season Can Be Serious
- At the Forefront in Fighting Cancer
- Molecular Motors: Think Small and yet Smaller Again…
- Liquid Biopsy: Novel Methods May Ease Cancer Detection and Therapy
- They Are Invisible, Sneaky and Disgusting – But Today It’s Their Special Day!
- How Many Cells Are in Your Body? Probably More Than You Think!
- What You Need to Know about Antibiotic Resistance – Findings, Facts and Good Intentions
- Why Do Old Men Have Big Ears?
- The Condemned Live Longer: A Potential Paradigm Shift in Genetics
- From Research to Commerce
- Chronobiology – How the Cold Seasons Influence Our Biorhythms
- Taskforce Microbots: Targeted Treatment from Inside the Body
- Eyes on Cancer Therapy
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- Challenges and Chances: A Review of the 1st Stem Cell Community Day
- Summertime, and the Livin’ Is Easy…
- Follow-on-Biologics – More than Simple Generics
- Bacteria Versus Body Cells: A 1:1 Tie
- Behind the Crime Scene: How Biological Traces Can Help to Convict Offenders
- Every 3 Seconds Someone in the World Is Affected by Alzheimer's
- HIV – It’s Still Not Under Control…
- How Many Will Be Convicted This Time?
- Malaria – the Battle is Not Lost
- Physicians on Standby: The Annual Flu Season Can Be Serious
- At the Forefront in Fighting Cancer
- Molecular Motors: Think Small and yet Smaller Again…
- Liquid Biopsy: Novel Methods May Ease Cancer Detection and Therapy
- They Are Invisible, Sneaky and Disgusting – But Today It’s Their Special Day!
- How Many Cells Are in Your Body? Probably More Than You Think!
- What You Need to Know about Antibiotic Resistance – Findings, Facts and Good Intentions
- Why Do Old Men Have Big Ears?
- The Condemned Live Longer: A Potential Paradigm Shift in Genetics
- From Research to Commerce
- Chronobiology – How the Cold Seasons Influence Our Biorhythms
- Taskforce Microbots: Targeted Treatment from Inside the Body
- Eyes on Cancer Therapy
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Regarding suitability of Eppendorf bioprocess equipment in GMP-regulated applications, please reach out to your Eppendorf sales representative.
Microbial Fermentation
Let's talk bacterial fermentation , let's talk yeast fermentation . Come talk to us, we are all ears.
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Eppendorf solutions for microbial fermentation
- Application flexibility: Cultivation of bacteria, yeasts, and other fungi under aerobic, microaerophilic, or anaerobic conditions
- Scalability: Scalable from 60 mL to 2400 L, Eppendorf offers a wide range of bioreactor control systems to help you scale up from the bench to industrial production
- Process optimization: Innovative hardware and software solutions to optimize process control, automate tasks, and harness the power of data
- Time-saving and risk mitigation: BioBLU® f Single-Use Bioreactors for microbial applications can be used as an option with most Eppendorf bioprocess systems to reduce the contamination risk and save setup time
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What properties make a bioreactor system suitable for microbial fermentation?
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Bioreactors should support high cell densities during aerobic fermentation
Oxygen supply: Essential for an aerobic cultivation to be successful
- Impellers: These are used to agitate and mix a cell culture. The number of blades, speed and diameter can all affect the DO level.
- Sparger: Spargers are tools for gas exchange, specifically for introducing air and oxygen. The type of sparger influences the created bubble size and how effectively oxygen can be transferred to the cell biomass.
- Gassing system: Gasses can be introduced into the bioreactor via the headspace or directly into the culture. This is called overlay and submerged gassing, respectively.
- Baffles: The use of baffles enhances the mixing process. It leads to more turbulent mixing and prevents the culture from vortex mixing by breaking the vortex.
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Culture feeding: Needed to encourage growth and microbial production
- Batch: Feeding substrate is only added at the beginning of the fermentation process.
- Fed-batch: Feeding substrate is added at the beginning and in increments throughout the rest of the bioprocess until the maximum working volume is reached.
- Continuous: Substrate is continuously added to the culture, with the continuous harvesting of cells and media to maintain a constant environment.
Fed-batch and continuous culture operation modes offer the possibility to incorporate feed automation and improve your fermentation process. Feed automation can be accomplished by tracking various parameters in real-time to keep track of the culture's metabolic health and automatically alter the substrate concentration with feed pumps. Bioprocess software like DASware® control can be utilized to automate feeding by monitoring important parameters in real-time.
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Temperature: Must be monitored and adjusted when necessary
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Bioprocess systems should facilitate process optimization
How parallel bioreactor systems can help accelerating process optimization
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How single-use bioreactors can help saving development time
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How to optimize bioprocess monitoring and control
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Bioreactors should support scale-up
How to scale-up your bioprocess
Process development is initially conducted using small volumes until the ideal conditions for microbial production have been found. A scaling up process is then carried out to reach the larger volumes required for production. This procedure is generally carried out in stages, from bench scale to pilot scale through to industrial scale. An important goal when scaling-up is to reproduce process performance across scales.
Bioprocess scale-up with the Eppendorf bioreactor systems
Scalable from 60 mL to 2,400 L, Eppendorf offers a wide range of bioreactor control systems to help you scale up from the bench to industrial production:
- Small-scale: The DASbox® Mini Bioreactor System and DASGIP® Parallel Bioreactor Systems cover working volumes of 60 mL to 1.8 L. They facilitate parallel operation of up to 24 bioreactors.
- Bench-scale: The SciVario® twin , BioFlo® 120 , and BioFlo® 320 cover working volumes of 250 mL to 3.8 L.
- Pilot/production-scale: The sterilize-in -place systems BioFlo® 510 , BioFlo® 610 , and BioFlo® Pro cover working volumes of 10.75 L to 2,400L.
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If needed, bioreactors should support microaerobic and anaerobic fermentation
Bioprocessing under anaerobic, microaerobic, and aerobic conditions
- Anaerobic: This describes an oxygen-free environment or a DO concentration of 0 %, and is commonly used in microbial production to manufacture various compounds including ethanol, lactic acid, and acetic acid.
- Micro-aerophilic: Conditions of less than 10 % DO are usually required for micro-aerobes, such as those found in the human gut. Probiotics are frequently produced using this type of fermentation.
- Aerobic: With a typical DO concentration of over 30 %, aerobic fermentation is essential for enhanced growth of many microbial cultures including E. coli.
For some organisms, their cultivation requires mimicking their natural environment. For example, in order to cultivate gut microbiome bacteria and their natural fermentation by-products, bioreactors need to be able to reproduce the microaerobic conditions found in the gastrointestinal tract. This requires fine control of DO levels through real-time monitoring and adjustment.
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Find out more
- Webinar: How to tailor your oxygen transfer rate (OTR)
- Application Note: Anaerobic Clostridium beijerinckii fermentation in Eppendorf bioreactor control systems
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How our customers use our products
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