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- Challenges and Chances: A Review of the 1st Stem Cell Community Day
- Summertime, and the Livin’ Is Easy…
- Follow-on-Biologics – More than Simple Generics
- Bacteria Versus Body Cells: A 1:1 Tie
- Behind the Crime Scene: How Biological Traces Can Help to Convict Offenders
- Every 3 Seconds Someone in the World Is Affected by Alzheimer's
- HIV – It’s Still Not Under Control…
- How Many Will Be Convicted This Time?
- Malaria – the Battle is Not Lost
- Physicians on Standby: The Annual Flu Season Can Be Serious
- At the Forefront in Fighting Cancer
- Molecular Motors: Think Small and yet Smaller Again…
- Liquid Biopsy: Novel Methods May Ease Cancer Detection and Therapy
- They Are Invisible, Sneaky and Disgusting – But Today It’s Their Special Day!
- How Many Cells Are in Your Body? Probably More Than You Think!
- What You Need to Know about Antibiotic Resistance – Findings, Facts and Good Intentions
- Why Do Old Men Have Big Ears?
- The Condemned Live Longer: A Potential Paradigm Shift in Genetics
- From Research to Commerce
- Chronobiology – How the Cold Seasons Influence Our Biorhythms
- Taskforce Microbots: Targeted Treatment from Inside the Body
- Eyes on Cancer Therapy
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Adapting Centrifugation Time
Lab Academy
What can you do if your centrifuge and rotor provide, e.g., only lower g × force than your protocol requires? You can centrifuge for an appropriate longer length of time. But how much longer? And how do you calculate this? The answer: by using the k-factor, a measure of the sedimentation distance. This will tell you how long it will take the particles to settle at the bottom of the test tube.
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The radius of the rotor and the angle of the bores as well as the type of vessel respectively the adapter you use will all have an impact on the sedimentation distance and thus on the centrifugation time you will need.
The k-factor is a measure of the sedimentation distance and tells us how long it will take until the particles settle at the bottom of the test tube.
The smaller the k-factor, the better the pelleting efficiency.
The formula for calculating the k-factor considers these parameters:
- Maximum rotor radius
- Minimum rotor radius
- Speed