JP | JPY
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- Summertime, and the Livin’ Is Easy…
- Follow-on-Biologics – More than Simple Generics
- Bacteria Versus Body Cells: A 1:1 Tie
- Behind the Crime Scene: How Biological Traces Can Help to Convict Offenders
- Every 3 Seconds Someone in the World Is Affected by Alzheimer's
- HIV – It’s Still Not Under Control…
- How Many Will Be Convicted This Time?
- Malaria – the Battle is Not Lost
- Physicians on Standby: The Annual Flu Season Can Be Serious
- At the Forefront in Fighting Cancer
- Molecular Motors: Think Small and yet Smaller Again…
- Liquid Biopsy: Novel Methods May Ease Cancer Detection and Therapy
- They Are Invisible, Sneaky and Disgusting – But Today It’s Their Special Day!
- How Many Cells Are in Your Body? Probably More Than You Think!
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- From Research to Commerce
- Chronobiology – How the Cold Seasons Influence Our Biorhythms
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- Eyes on Cancer Therapy
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- Challenges and Chances: A Review of the 1st Stem Cell Community Day
- Summertime, and the Livin’ Is Easy…
- Follow-on-Biologics – More than Simple Generics
- Bacteria Versus Body Cells: A 1:1 Tie
- Behind the Crime Scene: How Biological Traces Can Help to Convict Offenders
- Every 3 Seconds Someone in the World Is Affected by Alzheimer's
- HIV – It’s Still Not Under Control…
- How Many Will Be Convicted This Time?
- Malaria – the Battle is Not Lost
- Physicians on Standby: The Annual Flu Season Can Be Serious
- At the Forefront in Fighting Cancer
- Molecular Motors: Think Small and yet Smaller Again…
- Liquid Biopsy: Novel Methods May Ease Cancer Detection and Therapy
- They Are Invisible, Sneaky and Disgusting – But Today It’s Their Special Day!
- How Many Cells Are in Your Body? Probably More Than You Think!
- What You Need to Know about Antibiotic Resistance – Findings, Facts and Good Intentions
- Why Do Old Men Have Big Ears?
- The Condemned Live Longer: A Potential Paradigm Shift in Genetics
- From Research to Commerce
- Chronobiology – How the Cold Seasons Influence Our Biorhythms
- Taskforce Microbots: Targeted Treatment from Inside the Body
- Eyes on Cancer Therapy
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JP | JPY
Webinar
Optimize Your Plasmid Yields - Key Success Factors in Production from Shaker Flask to Fermenter
Ines Hartmann – Application Specialist Cell Handling Eppendorf SE Lab Academy
Recombinant plasmid DNA (pDNA) is produced in bacterial cultures, mostly in Escherichia coli (E. coli) in different scales from tube culture to fermenter. Successful pDNA production with consistent high yields and quality relies on multiple factors, including the vector type, the host strain, the insert, and the methodology chosen for cultivation and downstream purification.
In this webinar, we review the distinct steps in the plasmid production workflow and give tips on optimization: from vector and strain selection, to cultivation from shake flask to fermenter up to downstream processing like harvesting and purification. The focus will be on the optimization of the cultivation in shake flasks and fermenters.
Ines Hartmann is an application specialist for cell handling and responsible for the shakers and incubators at the Eppendorf headquarter in Hamburg, Germany. She joined the company in 2008 and has worked on several topics in cell handling, liquid handling and consumables. She obtained her diploma degree in biology at the Humboldt University of Berlin, Germany and gained expertise in various laboratory techniques.
Target groups:
- Scientists and technical personnel in academic and industrial setting, as well as students who start or currently work on plasmid production and want to improve their plasmid yields in tube, shake or fermenter cultures.
Key learning objectives:
- Learn about the different application fields of plasmid production
- Get an overview on the basic steps involved in plasmid production
- Learn what to consider in plasmid vector and host strain selection
- Learn how to optimize your plasmid culture in tube and shake flask culture
- Learn about stirred-tank fermenter culture set-up and scale-up considerations
- Get advice on harvesting and purification
- Live: “Ask the Expert” session – Deepen your knowledge and optimize your plasmid culture
Bio Speaker:
Ines Hartmann is an application specialist for cell handling and responsible for the shakers and incubators at the Eppendorf headquarter in Hamburg, Germany. She joined the company in 2008 and has worked on several topics in cell handling, liquid handling and consumables. She obtained her diploma degree in biology at the Humboldt University of Berlin, Germany and gained expertise in various laboratory techniques.
Register and watch recording now!
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