Can the Multipette/Repeater E3/E3x be used in a cold room?

The Multipette/Repeater E3/E3x can be used in a cold room down to 5°C. Please note that the technical data for imprecision and inaccuracy only apply to the temperature range 20 - 25 °C.

What is the difference between Multipette/Repeater E3 and Multipette/Repeater E3x?

The Multipette/Repeater E3 has three functions: dispensing, automatic dispensing and pipetting. The Multipette/Repeater E3x has four additional functions: sequential dispensing, aspiration, aspiration/dispensing, and titration. Both models use Combitips advanced.

Is it possible to change the dispensing speed?

Yes. The dispensing speed and the aspirating speed can be set to 8 different speed levels.

How long does it take to recharge the battery?

Charging takes approximately two hours. It is possible to go on working while charging. The lithium-ion battery used has no battery memory effect.

How large is the capacity of the fully charged battery?

The lithium-ion battery allows a minimum of ten thousand dispensing steps or the filling of more than hundred 96 well plates with a 10 mL Combitip ® advanced in a row.

Are the Multipette/Repeater E3/E3x stable to UV light?

The Multipette/Repeater E3 and E3x are stable to UV light. In the event of prolonged irradiation with UV light, material discolorations may occur, but this does not affect the proper function of the Multipette/Repeater.

How can the Multipette/Repeater be calibrated?

To calibrate Multipettes/Repeaters in accordance with EN ISO 8655, three different volumes with ten measured values each have to be checked. These three volumes are the nominal volume (= the maximum volume to be dispensed), 50% of the nominal volume and 10% of the nominal volume of the Combitips advanced used. The checking procedure, the test conditions, and the limits in accordance with EN ISO 8655 are described in our "Eppendorf SOP for checking pipettes". The SOP is available on our web page.

What is the function of the aspirate and dispense mode?

Mode A/D allows the aspiration of an unknown volume and its subsequent dispensing in equal parts. The usage of the A/D mode is advantageous for several applications in protein biochemistry, molecular biology, cell biology, and microbiology. Detailed information on different possible applications is given in White Paper No. 25.

What is the function of the selection dial position options (opt)?

In options, different parameters can be edited. These are: Keylock, favorites, volume, brightness, language, personalization, interval, date and time, and screen saver.

How do I determine the volume values when calibrating gravimetrically?

The measuring values of the analysis scales are multiplied by the correction factor Z (see SOP pipettes). This takes into account the density of the water at the relevant temperature and the relevant air.

What does EN ISO 8655 cover?

ISO 8655 addresses the needs of suppliers, test houses, and users and specifies the general requirements for piston-operated liquid handling devices. It is structured in seven chapters: ISO 8655-1: Terminology, general requirements, and user recommendations ISO 8655-2: Piston pipettes ISO 8655-3: Piston burettes ISO 8655-4: Dilutors ISO 8655-5: Dispensers ISO 8655-6: Gravimetric method for the determination of measurement error ISO 8655-7: Non-gravimetric methods for assessment of equipment performance (titration, photometry)

What do the terms random error and systematic error mean in a pipette calibration?

The systematic error describes the deviation of the dispensed volume from the nominal volume or from the selected volume of the piston stroke device, and is determined by establishing the mean value of (usually) 10 measurements. The random error describes the mean variation of the dispensed volumes from the mean value of the dispensed volumes, and is determined by calculating the repeat standard deviation of (usually) 10 measurements.

Why are the Z factors listed in our SOP different from factors which can be derived from density reference values (of water)?

The Z factor is included in EN ISO 8655-6 and was incorporated into our SOP. As we understand it, it was incorporated in the standard by the Physikalisch Technische Bundesanstalt in Braunschweig. The Z factor describes the density of water at a particular temperature using a 5th degree polynomial. Furthermore, the density of water is corrected by the density of air (derived from air pressure) and a fixed correction value for balances. These corrections are the key reasons for the deviation from density values in various books of tables.

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Multipette^® E3/Multipette^® E3x - 電子マルチディスペンサーピペット

Multipette® E3x electronic multi-dispenser by Eppendorf

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製品情報

Multipette E3/E3x 電動連続分注器を使用すると、液体の特性に関係なく、簡単かつ正確に分注できます。このリピーターピペットは軽量で操作が簡単なので、プレートや長い一連のチューブに負担をかけずに充填できます。 Combitips^® advancedの分注チップとViscoTip^®の組み合わせで、あらゆる液体ハンドリングの理想的なパートナーとなります。

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